Prepared By: Shruti Vij (Senior Analyst) , Geeta Mathur(Senior Scientist) ,Khushbu ( Analyst) This slide show contains detailed explanation of three characteristics of method validation- Limit of detection, Quantitation limits and Robustness. Limit of detection is the minimum amount of substance that can be detected but not measured, quantitation limit is the minimum amount of substance which can be detected and measured. Common approach to these procedures- signal to noise ratio has also been covered. Robustness is a characteristic which determines a method’s reliability when deliberate variations are induced in parameters.