This slide shows about the relationship between the genomic sequence of lactic acid bacterium and one of the idol character "KANA IMAI" in Idol Master Cinderella Girls.

1. LG21: lactic acid
bacterium
contains the idol,
"KANA IMAI"?
2017/10 Meteor★≡P（@cometwave）

2. Kana Imai
Japanese char: 今井加奈
height: 153cm
weight: 41kg
birth: March 3rd.
blood type: O
home village：Kochi (高知)
favorite: talk with friends
age: 16
measurements: 81/56/79
zodiac: Pisces
dominant hand: right
One of the pretty cute idol character
in the Japanese famous video game,
"Idol Master Cinderella Girls".
Kana Imai (N)

3. Congratulation for Kana's 21st
place in 6th general election!!!
Let's propose a toast with
the beverage, "LG21"!
[Memorial days]
Kana Imai (SSR+)

4. What's the LG21?
LG21 is a bacterial strain which is discovered from the library of
approximately more than 2500 strains of lactic acid bacterium in Meiji Co.,
Ltd. It is said that the extract of this bacterial body is good for your gut
health. The formal nomenclature of LG21 is "Lactobacillus gasseri OLL
2716". We picked up the initial letter, "L" and "G". We also picked up the
number "2" and "1", hoping for our jump toward the 21th century.
Translated from the website of Meiji co., Ltd.
http://www.meiji.co.jp/dairies/yogurt/lg21/#about
Wow! The product of new lactic acid bacterium!
Good job! Meiji!
But your abbreviation was intrepidity! ;D
[Memorial days]
Kana Imai (SSR)

5. By the way, the full genome sequence of
close strain of LG21 was reported in the
summer of 2006.
www.pnas.org/cgi/doi/10.1073/pnas.0607117103
Compared with the genomes of other lactic
acid bacterium, the rate of transposon
sequence was obviously small in this strain.
[Tropical vacation] Kana Imai(SR)

6. By the way again, the name,
"Kana Imai" has a special
feature as a sequence.
今井加奈
いまいかな
IMAI KANA
[Like a bad girl] Kana Imai(SR)

7. Yes! All of the letter can translate into
the abbreviated letter of proteinogenic
amino acid.
IMAI KANA
Isoleucine
Methionine
Alanine
Isoleucine Lysine
Alanine
Asparagine
Alanine
????
????
????
????
?
?
[Like a bad girl] Kana Imai(SR+)

8. So, the all nucleic acid sequence
have a probability for translating into
"IMAIKANA"!
ex)
ATTATGTATATTAAAGCTAATGCT
I M A I K A N A
[Friendly cheer] Kana Imai(SR+)
????
????
????
????
?
?

9. I want to feel like the existence of "Kana"
from the sequence of "IMAIKANA" in various
sequence of organism...
Let's search "IMAIKANA" with the BLAST
program from nr/nt sequence database!
[Chorus leader]
Kana Imai (SR)

10. Holy shit, no hit!
You ruined everything.
And nobody loves you?
NOOOOOOOOOOOOOOOOO!!!
[Dropping smile]
Kana Imai(SR)

11. Divide first name(Kana) and family
name(Imai), and let's search into
database with each sequence.
[Christmas party] Kana Imai(R)

12. …there it is!
[Dropping smile] Kana Imai(SR+)

13. Although they are a little bit separated…
I M A I
K A N A
two sequences were there!
[Friendly cheer]
Kana Imai(SR)

14. What is the source organism
of this sequence?
I felt like the existence of "KANA" :o
[Fancy girl] Kana Imai(SR)

15. Lactobacillus gasseri
ATCC 33323
It is the genomic sequence of the lactic
acid bacterium, which has been fully
analyzed in the summer of 2006!
[Tropical vacation] Kana Imai(SR+)

16. The genomic sequence of LG21 have
not been analyzed. But I believe it would
have the sequences of "KANA" and
"IMAI" because the close relative have
had them.
[Fancy girl]
Kana Imai(SR+)

17. END
[Flower garden] Kana Imai(R)

18. FAQ
Q: Are you joking？
A: Yes, I'm joking for fun, though it was a truth.
Q: The number of abbreviated letter of proteinogenic amino acid is 20, most of
alphabet letter. I guess, most of the idol would be translated from gene sequence...
A: Vowels of "U" and "O", frequently used in Japanese name, are not adopted in
proteinogenic amino acids. Therefore, a lot of idols are excluded
(ex: UZUKI, SHIBUYA, MIO).
Q: The amino acid sequences, "KANA" and "IMAI" is relatively short with the genomic
sequence. Would it be observed in the genomic sequence frequently?
A: If the existence probability of nucleic acid were equivalent,
the rate of "IMAI" is 1/465993, and the rate of "KANA" is 1/786432.
Hence, the existence probability of "IMAI" and "KANA" is...
1/465993 * 1/786432 = 1/366471806976
One case par 366Gbp. This probability is so small.
The length of LG genomic sequence was 1.9Mbp. So it was a kind of miracle!
Q: Are these genomic sequences correctly translated into IMAI and KANA?
A: I'm sorry, I didn't check the frame of translating.
Q: Are "IMAI" and "KANA" the coding regions?
A: * IMAI region = thiol reductase thioredoxin * KANA region = hypothetical protein
Q: So, what's your purpose?
A: I wanted to feel like the existence of KANA. I wanted everyone to know the idol KANA.
The voice actor of KANA has not been allowed yet. If you are interested in KANA,
and have a chance to vote in general election of idol master, please vote KANA!

19. Materials
#For the sequence analysis of KANA IMAI
*TBLASTN 2.7.30+
https://blast.ncbi.nlm.nih.gov/Blast.cgi
* Translated Query-Translated Subject BLAST 2.2.30+
ftp://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/2.2.30/
#Genomic sequence of Lactobacillus gasseri ATCC 33323
https://www.ncbi.nlm.nih.gov/nuccore/NC_008530.1
Thank you!
[Chorus leader]
Kana Imai(SR+)
If you are interested in
"Kana Imai", please access here!
->Im@s wiki
->Pixiv
->Idol master Cinderella girls
starlight stage (official)
->Idol master Cinderella girls
(official)
And sorry for my poor English…Xp