spot questions, clinical problems, instruments, techniques

1. Biochemistry Quiz- 3
Biochemistry For Medics
www.namrata.co
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2. Identify the disease
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3. Answer
• Palmar Xanthomas
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4. Breath Hydrogen
• Breath Hydrogen test is done for the
diagnosis of-------------------------- ?
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5. Answer
• Lactose intolerance
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6. Identify the structure and
mention its use
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7. Answer
• Biotin
• Participates in “Carboxylation reactions”
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8. Identify the technique and
mention its use
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9. Answer
• DNA foot printing
• For identification of promoter regions on
DNA
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10. Identify the instrument
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11. Answer
• Blood Gas Analyzer
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12. What is the normal reference
range for CK?
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13. Answer
• 10-50 IU/L
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14. Name the enzyme for the
second reaction
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15. Answer
• UDP –Glucuronyl Transferase
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16. What is the underlying
defect?
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17. Answer
• Its Trousseau‟s sign, an indication of latent
tetany
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18. What do you expect the
plasma concentration of
cholesterol in this patient ?
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19. Answer
• Higher than normal
• It‟s a case of Hypothyroidism.
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20. Which type of fatty acid is used
for esterification of cholesterol
by ACAT ?
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21. Answer
• Mono unsaturated fatty acid
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22. Name one precipitating factor
for the disease
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23. Answer
• Alcohol
• It is gouty arthritis
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24. Ribozyme ???
• Give one example of RNA acting as an
enzyme.
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25. Answer
• Peptidyl Transferase in translation
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26. Use of AMP ?
• Give an example where AMP part of ATP
is incorporated in to the substrate.
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27. Answer
• Conversion of FMN to FAD
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28. What is the significance of
red line in this diagram?
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29. Answer
• It indicates shine Delgarno sequence
• The small subunit/IF3 complex binds to the mRNA.
Specifically, it binds to the sequence AGGAGG,
known as the Shine-Delgarno sequence, which is
found in all prokaryotic mRNAs.( Its required for the
initiation of protein synthesis)
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30. RNA editing ? .
The central dogma states that for a given gene and gene
product there is a linear relationship between the coding
sequence in the DNA, the m- RNA sequence, and the
protein sequence.
Is there any exception to the rule if so then give
example and signify the purpose of this change ?
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31. Answer
• Editing of Apo – B m- RNA in the intestine
• Apo B 48 is a component of chylomicrons
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32. IDENTIFY THE INSTRUMENT
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33. Answer
• HPLC- High Performance Liquid
Chromatography for the separation or
purification of Biomolecules.
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34. MEGX
What is the full form ?
What is the significance ?
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35. Answer
1. MEGX Test (Mono ethyl Glycine
xylidine) is a liver function test based on
detoxifying functions of liver.
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36. What will the effect on Renal
Glutaminase activity in this
patient ?
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37. Answer
• It is signifying Respiratory Acidosis. Kidneys will tend to conserve
bicarbonates and excretion of H+ will increase, thus the glutaminase
activity will increase.
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38. Which complex in Electron
Transport Chain does not
have the Fe S center?
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39. Answer
Complex IV- (Cytochrome a-a3 oxidase
complex)
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40. PROBLEM
• The hypothetical „stimulin‟ gene contains two
exons that encode a protein of 100 amino acids.
They are separated by an intron of 100 bp
beginning after the codon for amino acid 10.
Stimulin messenger RNA has 5‟ and 3‟
untranslated regions of 70 and 30 nucleotides
respectively. A complementary DNA made from
mature stimulin RNA would have how many bp?
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41. Answer
• 400 bp
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42. Problem
• Which of the following involves
Isoprenoids ?
• a) The chromophore of visual pigment
• b) Carnitine
• c) Thiamine
• d) Ketone bodies
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43. Answer
a) The chromophore of visual pigment
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44. Problem
• What is the defect in Hereditary Non
polyposis colon cancer (HNPCC)?
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45. Answer
• Faulty mismatch repair
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46. Problem
• Name one molybdenum requiring
enzyme.
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47. Answer
• Xanthine oxidase
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48. Problem
• Chitin is a polymer of ?
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49. Answer
• The repeating unit in Chitin is N-Acetyl
Glucosamine.
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50. What is this structure?
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51. Answer
• β meander- Super secondary protein
structure
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52. What is the role of this vitamin in
Glycogen metabolism ?
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53. Answer
• It acts as a prosthetic group for the Muscle
Phosphorylase enzyme.
• So helps in muscle glycogenolysis.
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54. What is the Reichert- Meissl
Number for Butter ?
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55. Answer
• Butter has high Reichert- Meissl Number
and is the only fat with high number
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