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WORM WISDOM
Gut parasites are an age-
old problem that require
new solutions.
American Consortium for Small Ruminant
Parasite Control (ACSRPC)
Formed in response to the critical state of the small ruminant industry associated with the emergence
of anthelmintic resistant worms, the consortium is a group of scientists, veterinarians, and extension
specialists devoted to (1) developing novel methods for sustainable control of gastro-intestinal
nematodes in small ruminants and (2) educating the stakeholders in the small ruminant industry on
the most up-to-date methods and recommendations for control of gastrointestinal nematodes.
wormx.info or acsrpc.org
Online FAMACHA© Certification
• Two members of the consortium are
piloting online FAMACHA© certification.
• Dr. Katherine Petersson, University of Rhode Island
• Dr. Anne Zajac, Virginia-Maryland Regional College of Vet Medicine
1. View 2.5 hours of video: Integrated
Parasite Control and Why and How to Do
FAMACHA© scoring.
2. Make a video of yourself demonstrating your
FAMACHA© technique and send to URI.
3. Have follow-up with URI, if needed.
4. Upon completion receive certificate of
competence and right to purchase FAMACHA©
card(s).
http://web.uri.edu/sheepngoat/famacha/
Overview of problem
• Internal parasites are the primary
health problem affecting small
ruminants.
• Internal parasites can be a major
obstacle to profitability in many
production situations.
• Sheep and especially goats are more
susceptible to the effects of parasitism
than other farm livestock.
• Lack of FDA-approved drugs and
declining drug efficacy make parasite
control an even greater challenge.
Two parasites are usually of primary concern.
HAEMONCHUS CONTORTUS
Barber pole worm COCCIDIA (EIMERIA SPP.)
Effective parasite control requires an
integrated approach
Management (mostly)
• Pasture rest and rotation
• Forage management
• Host immunity
• Nutrition
• Timing of lambing
• Zero grazing (dry lot)
• Genetic selection
Drug (as needed)
• Maintain refugia
• Targeted selective treatment
• Test for drug resistance
• Combination treatments
These ain’t your father’s parasites!
Worms have developed resistance to all
dewormers and dewormer classes.
• Resistance is the genetic ability of a
worm to survive a dose of anthelmintic
which would normally be effective.
• Only worms that survive treatment
carry genes that confer resistance.
• Resistance is result of selection
through exposure of worm population
to an anthelmintic.
• When more than 5% of worms are
“drug tolerant”; i.e. failure to reduce
FEC by 95% or more (some say 90%).
http://www.scops.org.uk/what-is-resistance.html
Two ways to determine anthelmintic resistance
Fecal egg count reduction test (FECRT) DrenchRite® larval development assay
Fecal egg count reduction test (FECRT)
• Compare before and after fecal egg
counts
• Need ~15 animals per drug
1-2 animals won’t tell you much
• Need minimum FEC of 250 epg,
preferably higher.
• Can use individual or composite
(pooled) samples.
• Cost varies. Can learn to do yourself.
• “Logistically challenging”
DrenchRite® test
Larval development assay (LDA)
• Laboratory test that determines
resistance to all drug classes
simultaneously from a single pooled
fecal sample (~10 animals).
• Need minimum FEC of 500 epg.
• Also identifies worm species from
larvae, i.e. Percent Haemonchus
• Test done exclusively by Dr. Ray
Kaplan’s lab at University of Georgia.
• $450 per sample
Animal Before After % FECR
1 1000 100 90%
2 500 25 95%
3 6000 150 98%
4 4350 250 94%
5 3000 1000 67%
6 1200 400 67%
7 1500 200 87%
8 750 50 93%
9 1100 100 91%
10 3100 200 94%
11 2900 200 93%
12 475 200 58%
13 900 100 89%
14 1100 50 95%
15 300 0 100%
Avg 1878 145 87%
Fecal egg count reduction test (FECRT)
Anthelmintic resistance in southeastern US
29 farms in MD, VA, and GA (2016-2017, ASI Let’s Grow Project)
Determined by DrenchRite® test
0
10
20
30
40
50
60
70
80
90
100
Benzimidazoles Ivermectin Levamisole Moxidectin
Maryland Virginia Georgia
Percent farms with resistance
<95% FECR
Anthelmintic resistance in Michigan
7 large sheep farms (2013)
Determined by fecal egg count reduction test
0
20
40
60
80
100
Benzimidazoles Ivermectin Moxidectin Levamisole
Percent farms with resistance
<95% FECR
Resistance is inevitable!
Practices which accelerate development of resistant parasites:
1. Frequent deworming, especially calendar-based treatments
2. Whole flock/herd treatments
3. Under-dosing of drugs
4. Treat and move strategy: moving treated animals to a clean pasture
5. Rotating dewormers
6. Depositing drug in mouth instead of oral cavity
7. Use of persistent (long) activity dewormers (macrocylic lactones),
including pre-lambing drenching.
8. Use of injectable dewormers
9. Use of pour-on dewormers
10. Feeding dewormers to groups of animals
11. Improper storage of dewormers
Anthelmintics
(dewormers)
101
Anthelmintics 101
• An anthelmintic is a drug that expels
parasitic worms (helminths) and other
internal parasites from the body by
either stunning or killing them.
• It must be selectively toxic to the
parasite and not the host.
• It may be broad spectrum or targeted.
• It may contain single or multiple drug
actives.
Benzimidazoles were the first class of modern dewormers.
Anthelmintics 101
• Anthelmintics are separated into
classes (or groups) based on
similar chemical structure and
mode of action.
• While one drug may initially be
more potent than another, there
is cross-resistance among
anthelmintics in the same group.
• Anthelmintics for small
ruminants fall into three groups.
Ivermectin was introduced in the early 1980’s.
Anthelmintics for small ruminants
Benzimidazoles
Macrocylic lactones (MLs) Nicotinic agonists
Avermectins Milbemycins Imidazothiazoles Tetrahydropyrimidines
Thiabendazole
TBZ®
Ivermectin
Ivomec®
Moxidectin
Cydectin®
Quest®
Levamisole
Prohibit®
Leva-Med®
Tramisol®
Levasole®
Mortantel
Rumatel®
many trade names
Fenbendazole
SafeGuard®
Doramectin
Dectomax® Pyrantel
Strongid®
Albendazole
Valbazen®
Eprinomectin
Eprinex®
Oxfendazole
Synanthic®
1 2 3
FDA-approved anthelmintics for sheep
1
Benzimidazoles
VALBAZEN®
sheep drench
2a
Avermectins
IVOMEC®
sheep drench
2b
Milbimycins
CYDECTIN®
sheep drench
3
Levamisole
PROHIBIT®
LEVA-MED®
Adult worms    
Larvae (L4)    Limited
Hypobiotic larvae    Limited
Lungworms    
Tapeworms 
Liver flukes Adult stage
Coccidia
External parasites
Some
labeled for bot control
Some
Not labeled
Persistent activity  
Safety
10x
pregnancy restriction
(first 30 days)
20x 5x 3x
Labeled Dosage 3 ml/100 lbs. 3 ml/26 lbs. 1 ml/11 lbs.
Depends on dilution
2 ml/50 lbs.
(concentrated drench)
Meat withdrawal 7 days 11 days 7 days 3 days
FDA-approved anthelmintics for goats
1 Benzimidazoles 3b Morantel
Fenbendazole
SAFEGUARD®
Albendazole
VALBAZEN®
Feed premix
RUMATEL®
Adult worms  Not approved 
Larvae (L4)  Not approved sporadic
Hypobiotic larvae  Not approved
Lungworms  Not approved
Tapeworms Not labeled Not approved
Liver flukes Adult stage
Coccidia
External parasites
Persistent activity
Safety
wide 10x (sheep)
pregnancy restriction
~20x (sheep)
Labeled dosage 1.2 ml/50 lbs. 4 ml/100 lbs. Varies by product
Meat withdrawal 6 days 7 days 30 days
Milk withdrawal 0 days
Extra-label anthelmintics for goats
1 Benzimidazoles 2a Avermectins
IVOMEC®
sheep drench
2b Milbimycins
Moxidectin
CYDEDTIN® sheep drench
3a Levamisole
PROHIBIT®
LEVA-MED®
Fenbendazole
SAFEGUARD®
Albendazole
VALBAZEN®
Adult worms     
Larvae (L4)     Limited
Hypobiotic larvae     Limited
Lungworms     
Tapeworms Not labeled 
Liver flukes Adult stage
Coccidia
External parasites Some
label for bot control
Some
Not labeled
Persistent activity  
Safety
wide 10x
pregnancy restriction
20x 5x 3x
ACSRPC
recommended dosage
1.1 ml/25 lbs. 2 ml/25 lbs. 6 ml/25 lbs. 4.5 ml/25 lbs.
Depends on
dilution
Meat withdrawal 16 days
(1 day for each additional day used)
9 days 14 days 17 days 4 days
Milk withdrawal 4 days
(1 day for each additional day used)
7 days 9 days 8 days 3 days
“There now is very strong evidence
that using combination treatment
is the best method for using
dewormers and should be instituted
on all farms immediately.”
Dr. Ray Kaplan, University of Georgia (January 2017, www.wormx.info)
https://www.wormx.info/combinations
Rationale for combination treatments
• Most farms have resistance to at least
two dewormer groups.
• At first introduction, drug efficacy is over 99%.
• Once efficacy falls below 95%, drug resistance
is present, though drug is still useful for
treatment.
• As effectiveness of dewormer decreases
(<95%), as it is used more, it provides less and
less benefit to animals.
• Below 50%, it is no longer effective as the sole
treatment.
There is already resistance to Zolvix® in
other countries. Zolvix® is not sold in US.
Why give combination treatments?
• Contrary to popular belief, rotating between
dewormers will not prevent resistance from
developing. In fact, it will allow worms to
develop resistance to multiple drugs
simultaneously. It is no longer
recommended.
• Research done in New Zealand has shown
that the best approach is to use several
different dewormers at one time as a
combination treatment.
• When combined with “best management
practices” (that help to maintain refugia),
combination treatments may improve drug
efficacy and result in a reversion back toward
susceptibility.
Most dewormers sold in New Zealand and Australia
are combination products (multiple drug actives in
same product); No combination products are
available for small ruminants in US.
Why/How combination treatments work?
• Unlike rotating drugs, there is an additive
effect with each drug used in a
combination treatment.
• When resistance is low, there may be a
synergistic effect with a combination
treatment.
• By achieving a higher efficacy, there are
fewer resistant worms that survive
treatment.
• The sooner you start using combination
treatments the better, as you achieve the
greatest difference in the percentage of
resistant survivors when efficacy of
dewormers is high.
Drug 1 Drug 2 Drug 3 Combo12 Combo123
80% 80% 80% 96.00% 99.20%
90% 90% 90% 99.00% 99.90%
60% 95% 98.00% 98.00%
60% 60% 95% 84.00% 99.20%
99% 99% 99.99% 99.99%
60% 60% 60% 84.00% 93.60%
50% 50% 50% 75.00% 87.50%
40% 40% 40% 64.00% 78.40%
95% 80% 20% 99.00% 99.20%
Recommendations for combination treatments
• Purchase and administer each dewormer singly in
a separate syringe or drench gun.
• Do not mix dewormers.
• They are not chemically compatible.
• Only veterinarians have the right to compound
medications.
• Administer each dewormer at full dose based on
an accurate weight.
• Can give one drug immediately after the other.
• Observe withdrawal period of drug with longest
withdrawal period.
Recommendations for giving combo treatments
Valbazen®* Cydectin® Prohibit®*
Sheep 1.5 ml/50 lbs.
[7 days]
4.5 ml/50 lbs.
[7 days]
Depends on dilution
[3 days]
Goats 4 ml/50 lbs.
[9 days meat]
[7 days milk]
9 ml/50 lbs.
[17 days meat]
[8 days milk]
Depends on dilution
[4 days meat]
[3 days milk]
Camelids 4 ml/50 lbs. 9 ml/50 lbs. Depends on dilution
*Do not use first 30 days of pregnancy
www.wormx.info
Recommendations continued
• Do not give combination treatments to all
animals in a management group.
• Selective treat to maintain refugia.
1. FAMACHA© eye anemia system
2. Five Point Check©
3. Performance indicator(s)
• If you treat all animals, you will simultaneously
develop resistant to all dewormer classes,
eventually leaving you no drugs to effectively
treat clinically-parasitized animals.
• Combination treatments require extra-label
drug use in goats and camelids (VCPR).
S
S
S
S
S
S
S
S
S S
R
S
R
R R
R
S
S
R
Deworming
Copper oxide wire particles (COWPs)
can be successfully integrated into
Haemonchus contortus (barber pole
worm) management strategies on
sheep and goat farms, particularly
when producers are armed with
knowledge on how to use it safely.
Dr. Lisa Williamson
University of Georgia
Copper oxide wire particles (COWPs)
• Tiny needles of copper oxide.
• Slow release form of copper (Cu).
• Poorly absorbed form of copper vs. copper
sulfate which has caused copper toxicity 
when given to some sheep.
• COWPs have been shown to reduce barber
pole worm infections in sheep and goats.
Only “natural” substance with consistent
proven efficacy against worms in animal.
• Available as a copper supplement for cattle
(12, 25-g) and goats (2, 4-g). https://www.wormx.info/copper-oxide-wire-particles
Using COWPs to deworm small ruminants
1. FIRST: assess copper status of flock
(especially) or herd by submitting
livers to a lab for a mineral panel.
2. REPACKAGE cattle and goat boluses
into smaller doses for sheep and
goats.
3. ALWAYS use lowest dose possible to
achieve anthelmintic effect (more is
not better!).
• 0.5 to 1 g for lambs and kids
• 1-2 g for mature animals
MSU Veterinary Diagnostic Lab
https://www.animalhealth.msu.edu/
Using COWPs to deworm sheep and goats
1. SELECTIVELY treat: FAMACHA©
3s, 4s, and 5s.
2. ADMINISTER using a bolus or
balling gun; the gel cap is small,
so you may need something
sticky to hold capsule in the gun.
3. MINIMIZE the number of
treatments given to the same
animal.
4. PERIODICALLY check livers to see
what copper levels are. https://www.wormx.info/cowp-safety
Another combination treatment
COWPs + dewormer
Treatment
(10-23 lambs per Tx group)
Efficacy
(%FECR)
No treatment (control) Increase
Valbazen® (3 ml/50 lbs.) 20%
COWP (2 g, Ultracruz™) 58%
COWP (2 g, Copasure®) 12%
Valbazen® + COWP 99%
Burke, USDA-ARS, Booneville, Arkansas
http://www.wormx.info/cowpcombo
Similar results were obtained when
COWPs were combined with
levamisole (Prohibit®).
(USDA, unpublished data)
The concept of leaving
a proportion of animals
untreated, with the
aim to maintain
parasite populations in
refugia, is now widely-
accepted as the best
means of preserving
anthelmintic
susceptibility within
the parasite
population.
Treatment strategies for worm control
Suppressive 
• Whole-group
treatment at
regular
intervals.
Strategic 
• Whole-group
treatment at
strategic times,
such as before
parturition or
at weaning.
Targeted 
▪ Whole-group
treatment,
based on
marker of
infection.
Targeted selective
▪ Individual
treatment
based on
marker of
infection.
 REFUGIA BASED 
 CALENDAR BASED 
Targeted Selective Treatment (TST)
• Treating only a portion of the flock or
herd.
• Only treating animals that require or
would benefit from treatment.
• Requires having tools that can easily be
implemented on the farm, chute-side.
• Balance between delaying drug resistance
and maintaining productivity.
 

 




Decision-making tools for TST
1. FAMACHA© eye anemia system
2. Five Point Check©
3. Performance-based indicators
FAMACHA© eye anemia system
• A technique used in small ruminants to
assess the level of anemia resulting from
infection with Haemonchus contortus (the
barber pole worm).
• System developed in South Africa as an
alternative approach to the common practice
of frequent deworming of all animals, which
has been associated with widespread
emergence of anthelmintic resistance.
• Named for its originator: Faffa Malan (chart)
Dr. “Faffa” Malan (R)
FAMACHA© eye anemia system
Clinical
Category
Eye Lid
Color
Packed Cell
Volume/PCV
Treatment
recommendation
1 Red > 28 No
2 Red-Pink 23-27 No
3 Pink 18-22 ?
4 Pink-White 13-17 Yes
5 White < 12 Yes
Cards are now made in US.
University of Georgia is sole
distributor.
Proper FAMACHA© technique: COVER-PUSH-PULL-POP
1. COVER the animal’s eye with
your thumb.
2. Slightly PUSH down on the eye
ball, applying enough pressure so
that the eye lashes curl up over
your thumb.
3. After you PULL down the eye
lower eyelid,
4. The mucous membranes will POP
into view.
FAMACHA© DO’s and DON’Ts
DON’T
• Don’t try to expose
membranes simply by pulling
down eyelid.
• Don’t score inner surface of
lower eyelid; score bed of
mucous membranes.
• Don’t use half scores; round
up.
• Don’t shade eye
DO
• Repeat with other eye lid
• If you score the other eye
higher, err on the side of
caution and use the higher
score.
• Stick with first impressions.
• Score under natural lighting.
Deworming decisions with FAMACHA©
DEWORM FAMACHA© 4 and 5 DON’T DEWORM FAMACHA© 1 and 2*
*Unless there are other clinical signs of parasitism
Decision-making: deworm FAMACHA© 3s if . . .
• There is other evidence of parasitism.
• Flock or herd is not in good overall
body condition and health.
• More than 10% of flock/herd is
anemic (FAMACHA© 4, 5)
• There is a dramatic shift in scores.
• Periparturient females
• Lambs and kids
Proper care of FAMACHA© card
• Store FAMACHA© card in
dark place when not in use
• Replace after 12-24 months
of use
• Keep a spare card (unused)
for comparison of colors.
• Don’t try to reproduce card
by scanning and printing.
How to get a FAMACHA© card
• Veterinarians may purchase cards from
University of Georgia.
• Producers must take an approved training
in order to get a card.
• FAMACHA© certification is now available
online from the University of Rhode
Island.
http://web.uri.edu/sheepngoat/famacha/
Overcoming FAMACHA©’s limitations
It is limited to parasites that cause
anemia (blood loss)
Answer: Use Five Point Check©
It is labor intensive: each animal has to be
handled individually
Answer: Use Performance-based indicators
Five Point Check® 5.
• Simple extension of the FAMACHA© system,
which allows assessment of animal for other
parasites, especially those causing loss of
condition and weight and scours.
• Includes five check points on animal’s body.
1. Nose
2. Eye
3. Jaw
4. Back
5. Tail
# Checkpoint Observation Possibilities
1 Nose Nasal discharge 0-1
Nasal botfly
0–1 Lungworms
Pneumonia
Other diseases
2 Eye Anemia
1-5
FAMACHA© card
Barber pole worm
Liver fluke
Hook worms
Other diseases
3 Jaw Soft swelling 0-1
Barber pole worm
Liver fluke
Hook worms
Conical fluke
Other worms
Other diseases
4 Back Condition score
1-5
BCS card
Brown stomach worm
Bankrupt worm
(Long-necked bankrupt worm
Nodular worm
Tapeworms?
Other worms
Other diseases
5 Tail Soiling
0-1
Dag score card
Bankrupt worm
Conical fluke
Brown stomach worm
Nodular worm
Other worms
Other diseases
The Five Point
Check© for targeted
selective treatment of
internal parasites in
small ruminants
(Bath, 2009)
1. Nose
Snotty noses: a clear or
purulent nasal discharge may
be indicative of nasal bots.
Nasal bots are caused by the
bot fly, Oestrus ovis.
Infection is usually milder in
goats.
Ivermectin is highly effective against all stages of the larvae.
2. FAMACHA© score
3. Jaw
“Bottle jaw” Submandibular edema hypoproteinaemia
Accumulation of liquid under the jaw. Soft, cool, pitting edema. Swelling under jaw.
4. Back – body condition score
1 – emaciated
2 – thin
3 – average
4 – fat
5 – obese
Body condition scoring has many
uses on a sheep and goat farm.
It is an assessment of the amount of
muscle and fat covering the
backbone and the short ribs of each
animal.
Half scores are used.
5. Tail: fecal soiling
• Parasites which cause a decrease in body
condition score, may also cause mild to severe
diarrhea.
• Animals suffering from the worst diarrhea are
most likely to benefit from deworming.
• As with other check points, there are many
causes of diarrhea, but parasites is a major
one.
• It is proposed that only animals showing overt
signs of diarrhea be treated
What else should you consider when making
deworming decisions for FAMACHA© 3s.
• Frequency of scoring
• Hair coat condition
• Pot belly
• DISCO (diarrhea score)
Dry matter of feces
• Performance indicators
• Fecal egg count (FEC)
Performance-Based Indicators
• Non-Haemonchus parasites cause loss
of body weight and condition and
diarrhea (scours).
21% of farms in ASI Let’s Grow Study
to determine anthelmintic resistance
had < 60% Haemonchus contortus.
30
40
50
60
70
80
90
100
% HAEMONCHUS CONTORTUS
The Happy Factor™
• The Happy Factor™ decision-support model discriminates
between animals that are likely to respond favorably to
treatment vs. those that are not.
• Lambs which fail to reach target weights are dewormed; those
that do not, are not.
• Target weights are determined by modeling and are a measure
of nutrient efficiency: energy deposited ÷ energy consumed.
• Requires bi-weekly weighing; works best with precision-
farming technology.
• Happy Factor™ model results in less dewormer use, while
maintaining productivity.
Will the Happy Factor™ concept work in US?
• The Happy Factor™ has only been
evaluated in temperate climates
where non-Haemonchus worms
predominate (UK, NZ).
• In 2017, Vista View Farm
(Damascus, MD) conducted a SARE-
funded study to look at the
feasibility of using real-time
generated rate-of-gain to determine
deworming need where
Haemonchus is primary parasite
(97% according to larvae ID).
What Vista View Farm learned
• Rate-of-gain criteria (<0.3 lb/day for Bluefaced Leicester
lambs) was in agreement with FAMACHA© criteria (4 or 5)
70 percent of the time.
• It takes less time to weigh lambs as compared to
FAMACHA© scoring them (50% less w/RFID, auto-
weighing).
• Weekly weighing was too frequent
• Resulted in too much deworming
• Biweekly weighing is recommended
• There needs to be different rate-of-gain criteria for ewes
and wethers (and rams); challenge is coming up with gain
criteria.
What about using fecal egg counts as a decision
making tool for deworming?
• Not a highly accurate test, especially
at low numbers.
• Short pre-patent period of most
parasite makes FECs not very useful in
high challenge environments.
• No widely accept threshold for
treatment.
• Not farmer-friendly, not chute-side
What about using fecal egg counts as a
decision making tool for deworming?
• Not a reliable method to diagnose
parasitic disease in individual animal;
more accurate at the group level.
• Do not use fecal egg counts as a sole
indicator of when to deworm an animal.
• Can use fecal egg counts in conjunction
with FAMACHA©, Five Point Check®, or
other performance indicators.
Performance indicators for worm control
WEIGHT GAIN
• Lambs/kids
that fail to
meet
performance
targets are
dewormed.
MILK YIELD
• Higher-
producing dairy
females are
dewormed.
• For the same
reason, does
and ewes raising
multiples could
be targeted for
deworming.
• Also, yearlings.
BODY CONDITION
▪ A portion of the
flock/herd is left
untreated.
▪ The females in the
poorest body
condition are
dewormed.
FECAL EGG COUNT
▪ Deworm animals
when fecal egg
count reach a
certain threshold.
▪ Do fecal egg counts
on animals with
other indicators.
The fungus may soon be among us.
What is Duddingtonia flagrans?
• A natural strain of fungus isolated
from the environment and found
around the world.
• Nematophagous fungus
WORM-KILLING!
• Found on pasture (rarely in soil) or
in manure where it builds a
microscopic net that traps,
paralyzes, and consumes juvenile
stages (larvae) of parasitic worms.
How does it work?
1. It is a supplement fed to grazing livestock.
2. The spores pass through the digestive tract of the animals into the manure.
3. The fungus consumes larvae in the manure, preventing re-infection.
D. fragrans and worm control
• A non-chemical biological control
for parasites in grazing animals.
• Interrupts the crucial re-infestation
stage of the parasite’s life cycle,
thereby reducing re-infection of
contaminated pastures.
• Highly host-specific, only targeting
parasitic nematodes.
• Not harmful to the livestock.
"I have been told that the product should be
available in the US sometime early 2018. Stay tuned.
" Dr. Jim Miller, Louisiana State University (retired).
SUSAN SCHOENIAN
Sheep & Goat Specialist
sschoen@umd.edu
www.sheepandgoat.com
www.wormx.info

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Worm Wisdom

  • 1. WORM WISDOM Gut parasites are an age- old problem that require new solutions.
  • 2.
  • 3. American Consortium for Small Ruminant Parasite Control (ACSRPC) Formed in response to the critical state of the small ruminant industry associated with the emergence of anthelmintic resistant worms, the consortium is a group of scientists, veterinarians, and extension specialists devoted to (1) developing novel methods for sustainable control of gastro-intestinal nematodes in small ruminants and (2) educating the stakeholders in the small ruminant industry on the most up-to-date methods and recommendations for control of gastrointestinal nematodes.
  • 5. Online FAMACHA© Certification • Two members of the consortium are piloting online FAMACHA© certification. • Dr. Katherine Petersson, University of Rhode Island • Dr. Anne Zajac, Virginia-Maryland Regional College of Vet Medicine 1. View 2.5 hours of video: Integrated Parasite Control and Why and How to Do FAMACHA© scoring. 2. Make a video of yourself demonstrating your FAMACHA© technique and send to URI. 3. Have follow-up with URI, if needed. 4. Upon completion receive certificate of competence and right to purchase FAMACHA© card(s). http://web.uri.edu/sheepngoat/famacha/
  • 6. Overview of problem • Internal parasites are the primary health problem affecting small ruminants. • Internal parasites can be a major obstacle to profitability in many production situations. • Sheep and especially goats are more susceptible to the effects of parasitism than other farm livestock. • Lack of FDA-approved drugs and declining drug efficacy make parasite control an even greater challenge.
  • 7. Two parasites are usually of primary concern. HAEMONCHUS CONTORTUS Barber pole worm COCCIDIA (EIMERIA SPP.)
  • 8. Effective parasite control requires an integrated approach Management (mostly) • Pasture rest and rotation • Forage management • Host immunity • Nutrition • Timing of lambing • Zero grazing (dry lot) • Genetic selection Drug (as needed) • Maintain refugia • Targeted selective treatment • Test for drug resistance • Combination treatments
  • 9. These ain’t your father’s parasites!
  • 10. Worms have developed resistance to all dewormers and dewormer classes. • Resistance is the genetic ability of a worm to survive a dose of anthelmintic which would normally be effective. • Only worms that survive treatment carry genes that confer resistance. • Resistance is result of selection through exposure of worm population to an anthelmintic. • When more than 5% of worms are “drug tolerant”; i.e. failure to reduce FEC by 95% or more (some say 90%). http://www.scops.org.uk/what-is-resistance.html
  • 11. Two ways to determine anthelmintic resistance Fecal egg count reduction test (FECRT) DrenchRite® larval development assay
  • 12. Fecal egg count reduction test (FECRT) • Compare before and after fecal egg counts • Need ~15 animals per drug 1-2 animals won’t tell you much • Need minimum FEC of 250 epg, preferably higher. • Can use individual or composite (pooled) samples. • Cost varies. Can learn to do yourself. • “Logistically challenging”
  • 13. DrenchRite® test Larval development assay (LDA) • Laboratory test that determines resistance to all drug classes simultaneously from a single pooled fecal sample (~10 animals). • Need minimum FEC of 500 epg. • Also identifies worm species from larvae, i.e. Percent Haemonchus • Test done exclusively by Dr. Ray Kaplan’s lab at University of Georgia. • $450 per sample
  • 14. Animal Before After % FECR 1 1000 100 90% 2 500 25 95% 3 6000 150 98% 4 4350 250 94% 5 3000 1000 67% 6 1200 400 67% 7 1500 200 87% 8 750 50 93% 9 1100 100 91% 10 3100 200 94% 11 2900 200 93% 12 475 200 58% 13 900 100 89% 14 1100 50 95% 15 300 0 100% Avg 1878 145 87% Fecal egg count reduction test (FECRT)
  • 15. Anthelmintic resistance in southeastern US 29 farms in MD, VA, and GA (2016-2017, ASI Let’s Grow Project) Determined by DrenchRite® test 0 10 20 30 40 50 60 70 80 90 100 Benzimidazoles Ivermectin Levamisole Moxidectin Maryland Virginia Georgia Percent farms with resistance <95% FECR
  • 16. Anthelmintic resistance in Michigan 7 large sheep farms (2013) Determined by fecal egg count reduction test 0 20 40 60 80 100 Benzimidazoles Ivermectin Moxidectin Levamisole Percent farms with resistance <95% FECR
  • 17. Resistance is inevitable! Practices which accelerate development of resistant parasites: 1. Frequent deworming, especially calendar-based treatments 2. Whole flock/herd treatments 3. Under-dosing of drugs 4. Treat and move strategy: moving treated animals to a clean pasture 5. Rotating dewormers 6. Depositing drug in mouth instead of oral cavity 7. Use of persistent (long) activity dewormers (macrocylic lactones), including pre-lambing drenching. 8. Use of injectable dewormers 9. Use of pour-on dewormers 10. Feeding dewormers to groups of animals 11. Improper storage of dewormers
  • 19. Anthelmintics 101 • An anthelmintic is a drug that expels parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them. • It must be selectively toxic to the parasite and not the host. • It may be broad spectrum or targeted. • It may contain single or multiple drug actives. Benzimidazoles were the first class of modern dewormers.
  • 20. Anthelmintics 101 • Anthelmintics are separated into classes (or groups) based on similar chemical structure and mode of action. • While one drug may initially be more potent than another, there is cross-resistance among anthelmintics in the same group. • Anthelmintics for small ruminants fall into three groups. Ivermectin was introduced in the early 1980’s.
  • 21. Anthelmintics for small ruminants Benzimidazoles Macrocylic lactones (MLs) Nicotinic agonists Avermectins Milbemycins Imidazothiazoles Tetrahydropyrimidines Thiabendazole TBZ® Ivermectin Ivomec® Moxidectin Cydectin® Quest® Levamisole Prohibit® Leva-Med® Tramisol® Levasole® Mortantel Rumatel® many trade names Fenbendazole SafeGuard® Doramectin Dectomax® Pyrantel Strongid® Albendazole Valbazen® Eprinomectin Eprinex® Oxfendazole Synanthic® 1 2 3
  • 22. FDA-approved anthelmintics for sheep 1 Benzimidazoles VALBAZEN® sheep drench 2a Avermectins IVOMEC® sheep drench 2b Milbimycins CYDECTIN® sheep drench 3 Levamisole PROHIBIT® LEVA-MED® Adult worms     Larvae (L4)    Limited Hypobiotic larvae    Limited Lungworms     Tapeworms  Liver flukes Adult stage Coccidia External parasites Some labeled for bot control Some Not labeled Persistent activity   Safety 10x pregnancy restriction (first 30 days) 20x 5x 3x Labeled Dosage 3 ml/100 lbs. 3 ml/26 lbs. 1 ml/11 lbs. Depends on dilution 2 ml/50 lbs. (concentrated drench) Meat withdrawal 7 days 11 days 7 days 3 days
  • 23. FDA-approved anthelmintics for goats 1 Benzimidazoles 3b Morantel Fenbendazole SAFEGUARD® Albendazole VALBAZEN® Feed premix RUMATEL® Adult worms  Not approved  Larvae (L4)  Not approved sporadic Hypobiotic larvae  Not approved Lungworms  Not approved Tapeworms Not labeled Not approved Liver flukes Adult stage Coccidia External parasites Persistent activity Safety wide 10x (sheep) pregnancy restriction ~20x (sheep) Labeled dosage 1.2 ml/50 lbs. 4 ml/100 lbs. Varies by product Meat withdrawal 6 days 7 days 30 days Milk withdrawal 0 days
  • 24. Extra-label anthelmintics for goats 1 Benzimidazoles 2a Avermectins IVOMEC® sheep drench 2b Milbimycins Moxidectin CYDEDTIN® sheep drench 3a Levamisole PROHIBIT® LEVA-MED® Fenbendazole SAFEGUARD® Albendazole VALBAZEN® Adult worms      Larvae (L4)     Limited Hypobiotic larvae     Limited Lungworms      Tapeworms Not labeled  Liver flukes Adult stage Coccidia External parasites Some label for bot control Some Not labeled Persistent activity   Safety wide 10x pregnancy restriction 20x 5x 3x ACSRPC recommended dosage 1.1 ml/25 lbs. 2 ml/25 lbs. 6 ml/25 lbs. 4.5 ml/25 lbs. Depends on dilution Meat withdrawal 16 days (1 day for each additional day used) 9 days 14 days 17 days 4 days Milk withdrawal 4 days (1 day for each additional day used) 7 days 9 days 8 days 3 days
  • 25. “There now is very strong evidence that using combination treatment is the best method for using dewormers and should be instituted on all farms immediately.” Dr. Ray Kaplan, University of Georgia (January 2017, www.wormx.info) https://www.wormx.info/combinations
  • 26. Rationale for combination treatments • Most farms have resistance to at least two dewormer groups. • At first introduction, drug efficacy is over 99%. • Once efficacy falls below 95%, drug resistance is present, though drug is still useful for treatment. • As effectiveness of dewormer decreases (<95%), as it is used more, it provides less and less benefit to animals. • Below 50%, it is no longer effective as the sole treatment. There is already resistance to Zolvix® in other countries. Zolvix® is not sold in US.
  • 27. Why give combination treatments? • Contrary to popular belief, rotating between dewormers will not prevent resistance from developing. In fact, it will allow worms to develop resistance to multiple drugs simultaneously. It is no longer recommended. • Research done in New Zealand has shown that the best approach is to use several different dewormers at one time as a combination treatment. • When combined with “best management practices” (that help to maintain refugia), combination treatments may improve drug efficacy and result in a reversion back toward susceptibility. Most dewormers sold in New Zealand and Australia are combination products (multiple drug actives in same product); No combination products are available for small ruminants in US.
  • 28. Why/How combination treatments work? • Unlike rotating drugs, there is an additive effect with each drug used in a combination treatment. • When resistance is low, there may be a synergistic effect with a combination treatment. • By achieving a higher efficacy, there are fewer resistant worms that survive treatment. • The sooner you start using combination treatments the better, as you achieve the greatest difference in the percentage of resistant survivors when efficacy of dewormers is high. Drug 1 Drug 2 Drug 3 Combo12 Combo123 80% 80% 80% 96.00% 99.20% 90% 90% 90% 99.00% 99.90% 60% 95% 98.00% 98.00% 60% 60% 95% 84.00% 99.20% 99% 99% 99.99% 99.99% 60% 60% 60% 84.00% 93.60% 50% 50% 50% 75.00% 87.50% 40% 40% 40% 64.00% 78.40% 95% 80% 20% 99.00% 99.20%
  • 29. Recommendations for combination treatments • Purchase and administer each dewormer singly in a separate syringe or drench gun. • Do not mix dewormers. • They are not chemically compatible. • Only veterinarians have the right to compound medications. • Administer each dewormer at full dose based on an accurate weight. • Can give one drug immediately after the other. • Observe withdrawal period of drug with longest withdrawal period.
  • 30. Recommendations for giving combo treatments Valbazen®* Cydectin® Prohibit®* Sheep 1.5 ml/50 lbs. [7 days] 4.5 ml/50 lbs. [7 days] Depends on dilution [3 days] Goats 4 ml/50 lbs. [9 days meat] [7 days milk] 9 ml/50 lbs. [17 days meat] [8 days milk] Depends on dilution [4 days meat] [3 days milk] Camelids 4 ml/50 lbs. 9 ml/50 lbs. Depends on dilution *Do not use first 30 days of pregnancy www.wormx.info
  • 31. Recommendations continued • Do not give combination treatments to all animals in a management group. • Selective treat to maintain refugia. 1. FAMACHA© eye anemia system 2. Five Point Check© 3. Performance indicator(s) • If you treat all animals, you will simultaneously develop resistant to all dewormer classes, eventually leaving you no drugs to effectively treat clinically-parasitized animals. • Combination treatments require extra-label drug use in goats and camelids (VCPR). S S S S S S S S S S R S R R R R S S R Deworming
  • 32. Copper oxide wire particles (COWPs) can be successfully integrated into Haemonchus contortus (barber pole worm) management strategies on sheep and goat farms, particularly when producers are armed with knowledge on how to use it safely. Dr. Lisa Williamson University of Georgia
  • 33. Copper oxide wire particles (COWPs) • Tiny needles of copper oxide. • Slow release form of copper (Cu). • Poorly absorbed form of copper vs. copper sulfate which has caused copper toxicity  when given to some sheep. • COWPs have been shown to reduce barber pole worm infections in sheep and goats. Only “natural” substance with consistent proven efficacy against worms in animal. • Available as a copper supplement for cattle (12, 25-g) and goats (2, 4-g). https://www.wormx.info/copper-oxide-wire-particles
  • 34. Using COWPs to deworm small ruminants 1. FIRST: assess copper status of flock (especially) or herd by submitting livers to a lab for a mineral panel. 2. REPACKAGE cattle and goat boluses into smaller doses for sheep and goats. 3. ALWAYS use lowest dose possible to achieve anthelmintic effect (more is not better!). • 0.5 to 1 g for lambs and kids • 1-2 g for mature animals MSU Veterinary Diagnostic Lab https://www.animalhealth.msu.edu/
  • 35. Using COWPs to deworm sheep and goats 1. SELECTIVELY treat: FAMACHA© 3s, 4s, and 5s. 2. ADMINISTER using a bolus or balling gun; the gel cap is small, so you may need something sticky to hold capsule in the gun. 3. MINIMIZE the number of treatments given to the same animal. 4. PERIODICALLY check livers to see what copper levels are. https://www.wormx.info/cowp-safety
  • 36. Another combination treatment COWPs + dewormer Treatment (10-23 lambs per Tx group) Efficacy (%FECR) No treatment (control) Increase Valbazen® (3 ml/50 lbs.) 20% COWP (2 g, Ultracruz™) 58% COWP (2 g, Copasure®) 12% Valbazen® + COWP 99% Burke, USDA-ARS, Booneville, Arkansas http://www.wormx.info/cowpcombo Similar results were obtained when COWPs were combined with levamisole (Prohibit®). (USDA, unpublished data)
  • 37. The concept of leaving a proportion of animals untreated, with the aim to maintain parasite populations in refugia, is now widely- accepted as the best means of preserving anthelmintic susceptibility within the parasite population.
  • 38. Treatment strategies for worm control Suppressive  • Whole-group treatment at regular intervals. Strategic  • Whole-group treatment at strategic times, such as before parturition or at weaning. Targeted  ▪ Whole-group treatment, based on marker of infection. Targeted selective ▪ Individual treatment based on marker of infection.  REFUGIA BASED   CALENDAR BASED 
  • 39. Targeted Selective Treatment (TST) • Treating only a portion of the flock or herd. • Only treating animals that require or would benefit from treatment. • Requires having tools that can easily be implemented on the farm, chute-side. • Balance between delaying drug resistance and maintaining productivity.         
  • 40. Decision-making tools for TST 1. FAMACHA© eye anemia system 2. Five Point Check© 3. Performance-based indicators
  • 41. FAMACHA© eye anemia system • A technique used in small ruminants to assess the level of anemia resulting from infection with Haemonchus contortus (the barber pole worm). • System developed in South Africa as an alternative approach to the common practice of frequent deworming of all animals, which has been associated with widespread emergence of anthelmintic resistance. • Named for its originator: Faffa Malan (chart) Dr. “Faffa” Malan (R)
  • 42. FAMACHA© eye anemia system Clinical Category Eye Lid Color Packed Cell Volume/PCV Treatment recommendation 1 Red > 28 No 2 Red-Pink 23-27 No 3 Pink 18-22 ? 4 Pink-White 13-17 Yes 5 White < 12 Yes Cards are now made in US. University of Georgia is sole distributor.
  • 43. Proper FAMACHA© technique: COVER-PUSH-PULL-POP 1. COVER the animal’s eye with your thumb. 2. Slightly PUSH down on the eye ball, applying enough pressure so that the eye lashes curl up over your thumb. 3. After you PULL down the eye lower eyelid, 4. The mucous membranes will POP into view.
  • 44. FAMACHA© DO’s and DON’Ts DON’T • Don’t try to expose membranes simply by pulling down eyelid. • Don’t score inner surface of lower eyelid; score bed of mucous membranes. • Don’t use half scores; round up. • Don’t shade eye DO • Repeat with other eye lid • If you score the other eye higher, err on the side of caution and use the higher score. • Stick with first impressions. • Score under natural lighting.
  • 45. Deworming decisions with FAMACHA© DEWORM FAMACHA© 4 and 5 DON’T DEWORM FAMACHA© 1 and 2* *Unless there are other clinical signs of parasitism
  • 46. Decision-making: deworm FAMACHA© 3s if . . . • There is other evidence of parasitism. • Flock or herd is not in good overall body condition and health. • More than 10% of flock/herd is anemic (FAMACHA© 4, 5) • There is a dramatic shift in scores. • Periparturient females • Lambs and kids
  • 47. Proper care of FAMACHA© card • Store FAMACHA© card in dark place when not in use • Replace after 12-24 months of use • Keep a spare card (unused) for comparison of colors. • Don’t try to reproduce card by scanning and printing.
  • 48. How to get a FAMACHA© card • Veterinarians may purchase cards from University of Georgia. • Producers must take an approved training in order to get a card. • FAMACHA© certification is now available online from the University of Rhode Island. http://web.uri.edu/sheepngoat/famacha/
  • 49. Overcoming FAMACHA©’s limitations It is limited to parasites that cause anemia (blood loss) Answer: Use Five Point Check© It is labor intensive: each animal has to be handled individually Answer: Use Performance-based indicators
  • 50. Five Point Check® 5. • Simple extension of the FAMACHA© system, which allows assessment of animal for other parasites, especially those causing loss of condition and weight and scours. • Includes five check points on animal’s body. 1. Nose 2. Eye 3. Jaw 4. Back 5. Tail
  • 51. # Checkpoint Observation Possibilities 1 Nose Nasal discharge 0-1 Nasal botfly 0–1 Lungworms Pneumonia Other diseases 2 Eye Anemia 1-5 FAMACHA© card Barber pole worm Liver fluke Hook worms Other diseases 3 Jaw Soft swelling 0-1 Barber pole worm Liver fluke Hook worms Conical fluke Other worms Other diseases 4 Back Condition score 1-5 BCS card Brown stomach worm Bankrupt worm (Long-necked bankrupt worm Nodular worm Tapeworms? Other worms Other diseases 5 Tail Soiling 0-1 Dag score card Bankrupt worm Conical fluke Brown stomach worm Nodular worm Other worms Other diseases
  • 52. The Five Point Check© for targeted selective treatment of internal parasites in small ruminants (Bath, 2009)
  • 53. 1. Nose Snotty noses: a clear or purulent nasal discharge may be indicative of nasal bots. Nasal bots are caused by the bot fly, Oestrus ovis. Infection is usually milder in goats. Ivermectin is highly effective against all stages of the larvae.
  • 55. 3. Jaw “Bottle jaw” Submandibular edema hypoproteinaemia Accumulation of liquid under the jaw. Soft, cool, pitting edema. Swelling under jaw.
  • 56. 4. Back – body condition score 1 – emaciated 2 – thin 3 – average 4 – fat 5 – obese Body condition scoring has many uses on a sheep and goat farm. It is an assessment of the amount of muscle and fat covering the backbone and the short ribs of each animal. Half scores are used.
  • 57. 5. Tail: fecal soiling • Parasites which cause a decrease in body condition score, may also cause mild to severe diarrhea. • Animals suffering from the worst diarrhea are most likely to benefit from deworming. • As with other check points, there are many causes of diarrhea, but parasites is a major one. • It is proposed that only animals showing overt signs of diarrhea be treated
  • 58. What else should you consider when making deworming decisions for FAMACHA© 3s. • Frequency of scoring • Hair coat condition • Pot belly • DISCO (diarrhea score) Dry matter of feces • Performance indicators • Fecal egg count (FEC)
  • 59. Performance-Based Indicators • Non-Haemonchus parasites cause loss of body weight and condition and diarrhea (scours). 21% of farms in ASI Let’s Grow Study to determine anthelmintic resistance had < 60% Haemonchus contortus. 30 40 50 60 70 80 90 100 % HAEMONCHUS CONTORTUS
  • 60. The Happy Factor™ • The Happy Factor™ decision-support model discriminates between animals that are likely to respond favorably to treatment vs. those that are not. • Lambs which fail to reach target weights are dewormed; those that do not, are not. • Target weights are determined by modeling and are a measure of nutrient efficiency: energy deposited ÷ energy consumed. • Requires bi-weekly weighing; works best with precision- farming technology. • Happy Factor™ model results in less dewormer use, while maintaining productivity.
  • 61. Will the Happy Factor™ concept work in US? • The Happy Factor™ has only been evaluated in temperate climates where non-Haemonchus worms predominate (UK, NZ). • In 2017, Vista View Farm (Damascus, MD) conducted a SARE- funded study to look at the feasibility of using real-time generated rate-of-gain to determine deworming need where Haemonchus is primary parasite (97% according to larvae ID).
  • 62. What Vista View Farm learned • Rate-of-gain criteria (<0.3 lb/day for Bluefaced Leicester lambs) was in agreement with FAMACHA© criteria (4 or 5) 70 percent of the time. • It takes less time to weigh lambs as compared to FAMACHA© scoring them (50% less w/RFID, auto- weighing). • Weekly weighing was too frequent • Resulted in too much deworming • Biweekly weighing is recommended • There needs to be different rate-of-gain criteria for ewes and wethers (and rams); challenge is coming up with gain criteria.
  • 63. What about using fecal egg counts as a decision making tool for deworming? • Not a highly accurate test, especially at low numbers. • Short pre-patent period of most parasite makes FECs not very useful in high challenge environments. • No widely accept threshold for treatment. • Not farmer-friendly, not chute-side
  • 64. What about using fecal egg counts as a decision making tool for deworming? • Not a reliable method to diagnose parasitic disease in individual animal; more accurate at the group level. • Do not use fecal egg counts as a sole indicator of when to deworm an animal. • Can use fecal egg counts in conjunction with FAMACHA©, Five Point Check®, or other performance indicators.
  • 65. Performance indicators for worm control WEIGHT GAIN • Lambs/kids that fail to meet performance targets are dewormed. MILK YIELD • Higher- producing dairy females are dewormed. • For the same reason, does and ewes raising multiples could be targeted for deworming. • Also, yearlings. BODY CONDITION ▪ A portion of the flock/herd is left untreated. ▪ The females in the poorest body condition are dewormed. FECAL EGG COUNT ▪ Deworm animals when fecal egg count reach a certain threshold. ▪ Do fecal egg counts on animals with other indicators.
  • 66. The fungus may soon be among us.
  • 67. What is Duddingtonia flagrans? • A natural strain of fungus isolated from the environment and found around the world. • Nematophagous fungus WORM-KILLING! • Found on pasture (rarely in soil) or in manure where it builds a microscopic net that traps, paralyzes, and consumes juvenile stages (larvae) of parasitic worms.
  • 68. How does it work? 1. It is a supplement fed to grazing livestock. 2. The spores pass through the digestive tract of the animals into the manure. 3. The fungus consumes larvae in the manure, preventing re-infection.
  • 69. D. fragrans and worm control • A non-chemical biological control for parasites in grazing animals. • Interrupts the crucial re-infestation stage of the parasite’s life cycle, thereby reducing re-infection of contaminated pastures. • Highly host-specific, only targeting parasitic nematodes. • Not harmful to the livestock. "I have been told that the product should be available in the US sometime early 2018. Stay tuned. " Dr. Jim Miller, Louisiana State University (retired).
  • 70. SUSAN SCHOENIAN Sheep & Goat Specialist sschoen@umd.edu www.sheepandgoat.com www.wormx.info