one of human body fluids which is important in infertility assessment is seminal fluids .medical laboratories in Iran are responsible to analyse the semen and report back to specialist .the technicians responsible for this analyse are mostly microbiologists so I decided to teach my students the general information about semen analysis in this powerpoint.

1. Prepared by :
Dr. Afagh Bapirzadeh
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2. Importance of
infertility
• Every Country has a rate of
infertility and fertility which
matters for future planning
• Having a family with children
• Avoiding depression
• Being aware of ones body
condition
• Curing the diseases if possible
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• Primary infertility : regularly having
intercourse with your partner to get
to get pregnant over a year
(12months)
• Secondary infertility :you may have
one child but after 6 months of
of trying you cant conceive
Infertility definition byWHO

4. Infertility Male infertility
Female infertility
Unexplained
infertility
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5. Male infertility diagnosis
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6. Male reproductive
system parts
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7. spermatogenesis
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• Spermatogonia
• Primary spermatocytes
• Secondary spermatocytes
• Spermatids
• spermatozoa

8. Sperm macroscopic analysis
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9. What should you do in the lab
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1. Measure PH of the seminal fluids
2. Investigate the viscosity of the
semen
3. Let the sample liquefaction for 30
minutes
4. measure the sperm volume

10. Sperm microscopic analysis
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11. Parts of a normal sperm
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• Head
• Intermediate piece
• Tail
• Cytoplasmic content

12. Abnormal sperms in seminal fluids
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13. Sperm motility
types
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• Progressive motility (grade A)
• Non-progressive motility (grade B)
• Immotile (dead ones)

14. Sperm concentration in seminal fluids
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15. Computer Assisted Semen Analysis(CASA)
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16. Wet preparation of sperm analysis
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1. Place one drop of semen on a slide
2. Place a cover glass on it
3. Look under microscope with X100
4. Report what you see
5. Change your area at least 5 times
6. Count the sperms
7. Recognize the sperm shapes

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