Guidelines of CPCSEA

1. Presented by- Smita kapoor

2. • CPCSEA is a statutory Committee,
• established under Chapter 4, Section 15(1) of the
Prevention of Cruelty to Animals Act 1960.
• India is one of the pioneering countries to institute
Prevention of Cruelty to Animals Act in 1960.
• Detailed rules for experimentation on animals were first
enacted by the Ministry of Agriculture in 1968.
• Present Committee was constituted on 30th August,
2012
• At present, Shri Hem Pande, Additional Secretary,
MoEF&CC is the Chairman of the Committee.

3. To promote the humane care of animals used in
biomedical and behavioral research and testing.
to provide specifications that will enhance animal well
being.
To provide quality in gaining advanced biological
knowledge that is relevant to humans and animals

4. VETERINARY CARE
Adequate veterinary care must be provided and is
the responsibility of a veterinarian.
Daily observation.
ANIMAL PROCUREMENT
All animals must be acquired lawfully as per the
CPCSEA guidelines.
A health surveillance program for incoming animals
should be carried out to assess animal quality.
 Inspect for compliance with procurement
specifications.

5. • QUARANTINE
An effective quarantine minimizes the chance for introduction
of pathogens into an established colony.
A minimum duration of quarantine
- Small lab animals - 1 week and
- Larger animals - 6 weeks
• STABILIZATION AND SEPARATION
Newly received animals should be given a period for
physiologic, psychologic and nutritional stabilization before
their use.
Duration for stabilization will depend on the type of animal,
transportation and intended use.
Physical separation of animals by species is recommended.

6. • SURVEILLANCE, DIAGNOSIS, TREATMENT AND
CONTROL OF DISEASE
Observe for signs of illness, injury, or abnormal
behavior.
Unexpected deaths and signs of illness should be
reported.
If animals are known to be exposed to an infectious
agent the group should be kept intact and isolated
during the process of diagnosis, treatment, and control.
Diagnostic clinical laboratory may be made available.

7. • ANIMAL CARE AND TECHNICAL PERSONNEL
Employ people trained in laboratory animal science.
They should be provide for both formal and on-the-job
training.
• PERSONAL HYGIENE
It is essential to maintain a high standard of personal
cleanliness.
Decontaminate clothing exposed to potentially hazardous
microbial agents or toxic substances.
Use disposable gear.
No permission to eat, drink, smoke or apply cosmetics in
animal rooms.

8. • ANIMAL EXPERIMENTATION INVOLVING
HAZARDOUS AGENTS
Institutional Biosafety Committee.
The procedures must be reviewed by both the Institutional
Biosafety committee and Institutional Animal Ethics
Committee (IAEC).
• MULTIPLE SURGICAL PROCEDURES ON SINGLE
ANIMAL
Multiple surgical procedures not to be practiced unless
specified in a protocol only approved by the IAEC.
• DURATIONS OF EXPERIMENTS
No animal should be used for experimentation for more than
3 years unless adequate justification is provided.

9. • PHYSICAL RESTRAINT
Brief physical restraint can be accomplished manually or with
devices.
Prolonged restraint of any animal should be avoided unless
essential to research objectives.
Less restrictive systems, such as the tether system or the pole and
collar system should be used when compatible with research
objectives.
• The following are important guidelines for the use of restraint
equipments:
Not be used simply as a convenience in handling or managing
animals.
Minimum time.
Should be given training to adapt to the equipment.
Observe the animal at appropriate intervals.
Veterinary care should be provided if lesions or illness associated
with restraint are observed.

10. • PHYSICAL PLANT
The physical condition and design of animal facility hould be
well planned and properly maintained.
• PHYSICAL RELATIONSHIP OF ANIMAL
FACILITIES TO LABORATORY
Isolated far away from human habitation.
Place animal housing areas adjacent to or near laboratories
but separated.
• FUNCTIONAL AREAS
Sufficient animal area required to: Ensure separation of
species or isolation of individual projects when necessary;
Receive, quarantine, and isolate animals;
Provide for animal housing.

11. • PHYSICAL FACILITIES
(1)Building material
(2)Animal room doors
(3)Floors
(4)Drains
(5)Storage areas
(6)Experimental area
(7)Corridor
(8)Exterior windows
• ENVIRONMENT
(a) TEMPERATURE AND HUMIDITY CONTROL
 18 to 29°C temperature range3
0% to70% relative humidity
(b) VENTILATION

12. (c) POWER AND LIGHTING
A time-controlled lighting system.
Emergency power should be available in the event of power
failure
(d) NOISE CONTROL
• ANIMAL HUSBANDRY
• (a) CAGING OR HOUSING SYSTEM:
Provide adequate space
Comfortable environment
Escape proof enclosure that confines animal safety.
Easy access to food and water.
Provide adequate ventilation.
 Meet the biological needs of the animals.
Keep the animals dry and clean.

13. • FOOD
Animals should be fed palatable, non-contaminated, and
nutritionally adequate food daily.
• BEDDING
• DESIRABLE CRITERIA
Absorbent
Free of toxic chemicals
Ammonia binding
Sterilizable
Easily stored
Uncontaminated
Nontoxic
Disposable by incineration
Readily available

14. • WATER
Continuous access of fresh, potable, uncontaminated drinking
water.
Periodic monitoring of microbial contamination.
• SANITATION and CLEANLINESS
Cleaning with detergents and disinfectants.
Wire – bottom rodent cages should be washed at least every 2
weeks.
Disinfection of cages by rinsing at a temperature of 82.20C (180 F).
• WASTE DISPOSAL AND PEST CONTROL
Remove regularly and frequently.
Animal tissues, carcasses, and hazardous wastes should be lined
with leak – proof, disposable liners.
EMERGENCY, WEEKEND AND HOLIDAY CARE
Animals should be cared by qualified personnel every day, including
weekends and holidays.

15. • RECORD KEEPING
The Animal House should maintain following records:
• Animal House plans
• Animal House staff record
• All SOPs for animals
• Breeding, stock, purchase and sales records
• Minutes of institutional Animals Ethics Committee meetings
• Records of experiments
• Death Record
• Clinical record
• Water analysis report

16. • STANDARD OPERATING PROCEDURES (SOPs)
The Institute shall maintain SOPs describing procedures / methods of
• Animal Husbandry
• Maintenance
• Breeding
• Animal house microbial analysis
• Experimentation records.
• TRANSPORT OF LABORATORY ANIMALS
• The main considerations for transport of animals are:
• Mode of transport
• Containers
• Animal density in cages
• Food and water during transit
• Protection from transit infections
• Injuries and stress

17. OPERATIONALGUIDELINESINCPCSEA(10)
Main activities
(a) Registration of establishments for Breeding of animals:
(b) Registration of establishments for experiments on animals:
(c) Approval of Animal House Facilities:
(d) Permission of Committee for Conducting Experiments:
(e) Recommendation for import
• Regulatory Mechanism :
 Committee for the Purpose of Control and Supervision of Experiments on
Animals (CPCSEA)
• To ensure that animals are not subjected to unnecessary pain or suffering
before, during or after performance of experiments on them.
 Sub-Committees of the CPCSEA:
1) Sub-Committee on Large Animals (SCLA).
2) Sub-Committee for selection of CPCSEA Nominees on Institutional Animals
Ethics Committees.