Ankitha Hirematha

Bhadravati Area, India India

BCIL Trainee

• Animal cell culture : Preparation of media, media filtration, subculturing of monolayer, cell counting, MTT Assay, Preparation of cryopreservant, cryopreservation of cells, microcarrier cell culture, Giemsa staining, seeding of 5L bioreactor, harvesting cells from microcarrier and bioreactor. • Downstream processing : ultrafiltration, microfiltration, Affinity chromatography, Anion and cation Exchange chromatography, Gel filtration etc.. • Cleaning, sterilization and Filtration of Fermentor : Drawing P&Id, valve matrices from URS, CIP and SIP of filtration system, CIP and SIP of fermentors, Integrity testing of filters, DHS and autoclave Qualification. • Microbial Fermentatio...